SubtiBank SubtiBank
ansB [2018-02-13T02:50:14.000Z]
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ansB [2018-02-13T02:50:14.000Z]

L-aspartase
Locus
BSU23570
Isoelectric point
5.69
Molecular weight
52.39 kDa
Protein length
475 aa Sequence Blast
Gene length
1425 bp Sequence Blast
Function
aspartate degradation
Product
L-aspartase
Essential
no
E.C.
4.3.1.1
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of asparagine/ aspartate

    • Gene

    Coordinates
    2,454,347 → 2,455,774

    The protein

    Catalyzed reaction/ biological activity

  • L-aspartate = fumarate + NH3 (according to Swiss-Prot)

    • Protein family

  • Aspartase subfamily (according to Swiss-Prot)

    • Paralogous protein(s)

  • CitG

    • Structure

  • 3R6Q (from Bacillus sp. YM55-1, 72% identity, 92% similarity) PubMed

    • Expression and Regulation

    Operons

    Genes
    ansA-ansB
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • AnsR: repression, PubMed, in AnsR regulon

    • Regulation

  • expressed in the presence of asparagine (AnsR) PubMed
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    Genes
    ansA-ansB-mleN-mleA
    Description
    PubMed

    Regulation

  • ansA: expressed in the presence of asparagine (AnsR) PubMed
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    Biological materials

    Mutant

  • GP1153 (del ansAB::ermC) available in the Stülke lab
  • BKE23570 (ΔansB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATCTTTAACCTTCTTT, downstream forward: _UP4_TAAAATTTGTATAAAATACA
  • BKK23570 (ΔansB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATCTTTAACCTTCTTT, downstream forward: _UP4_TAAAATTTGTATAAAATACA

    • References

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